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In Vitro Propagation of Pink Lapacho : Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components
Joint Authors
Apóstolo, Nancy Mariel
Llorente, Berta Elizabet
Larraburu, Ezequiel Enrique
Source
International Journal of Forestry Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Handroanthus impetiginosus, pink lapacho, is a timber, ornamental, and medicinal tree.
Experiments on the in vitro propagation of H.
impetiginosus were conducted using nodal segments cultivated in both Murashige and Skoog salts with Gamborg vitamins (MSG) and Woody Plant Medium (WPM) with different concentrations of 6-benzylaminopurine (BA) and indole butyric acid (IBA).
Morphogenic responses were differentially affected by salt compositions and their interactions with plant growth regulators in each micropropagation stage.
According to response surface analysis, the optimum multiplication rate with 1 μM IBA ranged from 16.7 to 21.3 μM BA in WPM, and the inhibitors of endogenous auxins could increase multiplication rates.
A pulse with 50 μM IBA in 1/2 MSG produced 83% rooting with 3.2 roots per shoots and higher fresh and dry weights of shoots and roots.
In the acclimatisation stage, 50% of plants survived after 1 year.
This methodology optimised the culture media for the in vitro propagation of the H.
impetiginosus clonal pool and could be applied to related species, several of which are categorised as vulnerable on the International Union for the Conservation of Nature Red List.
American Psychological Association (APA)
Larraburu, Ezequiel Enrique& Apóstolo, Nancy Mariel& Llorente, Berta Elizabet. 2012. In Vitro Propagation of Pink Lapacho : Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components. International Journal of Forestry Research،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-463086
Modern Language Association (MLA)
Larraburu, Ezequiel Enrique…[et al.]. In Vitro Propagation of Pink Lapacho : Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components. International Journal of Forestry Research No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-463086
American Medical Association (AMA)
Larraburu, Ezequiel Enrique& Apóstolo, Nancy Mariel& Llorente, Berta Elizabet. In Vitro Propagation of Pink Lapacho : Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components. International Journal of Forestry Research. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-463086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463086