Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS
Joint Authors
Zhang, Ning
Wang, Fashan
Tan, Guanghui
Zhu, Kuiyuan
Ma, Wei
Zou, Taobo
Geng, Fang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established.
The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) under a gradient elution.
The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V).
Twelve absorbed compounds and four metabolites were found.
Seven of the absorbed compounds were identified by ESI-MS.
The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.
American Psychological Association (APA)
Geng, Fang& Wang, Fashan& Zou, Taobo& Zhu, Kuiyuan& Ma, Wei& Tan, Guanghui…[et al.]. 2013. Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-463134
Modern Language Association (MLA)
Geng, Fang…[et al.]. Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-463134
American Medical Association (AMA)
Geng, Fang& Wang, Fashan& Zou, Taobo& Zhu, Kuiyuan& Ma, Wei& Tan, Guanghui…[et al.]. Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-463134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463134