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Differential Dynamics of ATR-Mediated Checkpoint Regulators
Joint Authors
Warmerdam, Daniël O.
Smits, Veronique A. J.
Kanaar, Roland
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-17
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The ATR-Chk1 checkpoint pathway is activated by UV-induced DNA lesions and replication stress.
Little was known about the spatio and temporal behaviour of the proteins involved, and we, therefore, examined the behaviour of the ATRIP-ATR and Rad9-Rad1-Hus1 putative DNA damage sensor complexes and the downstream effector kinase Chk1.
We developed assays for the generation and validation of stable cell lines expressing GFP-fusion proteins.
Photobleaching experiments in living cells expressing these fusions indicated that after UV-induced DNA damage, ATRIP associates more transiently with damaged chromatin than members of the Rad9-Rad1-Hus1 complex.
Interestingly, ATRIP directly associated with locally induced UV damage, whereas Rad9 bound in a cooperative manner, which can be explained by the Rad17-dependent loading of Rad9 onto damaged chromatin.
Although Chk1 dissociates from the chromatin upon UV damage, no change in the mobility of GFP-Chk1 was observed, supporting the notion that Chk1 is a highly dynamic protein.
American Psychological Association (APA)
Warmerdam, Daniël O.& Kanaar, Roland& Smits, Veronique A. J.. 2010. Differential Dynamics of ATR-Mediated Checkpoint Regulators. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-16.
https://search.emarefa.net/detail/BIM-463151
Modern Language Association (MLA)
Warmerdam, Daniël O.…[et al.]. Differential Dynamics of ATR-Mediated Checkpoint Regulators. Journal of Nucleic Acids No. 2010 (2010), pp.1-16.
https://search.emarefa.net/detail/BIM-463151
American Medical Association (AMA)
Warmerdam, Daniël O.& Kanaar, Roland& Smits, Veronique A. J.. Differential Dynamics of ATR-Mediated Checkpoint Regulators. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-16.
https://search.emarefa.net/detail/BIM-463151
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463151