Effects of Canonical NF-κB Signaling Pathway on the Proliferation and OdontoOsteogenic Differentiation of Human Stem Cells from Apical Papilla
Joint Authors
Jing, Shuanglin
Yu, Jinhua
Li, Yao
Liu, Genxia
Wang, Zilu
Yan, Ming
Li, Junjun
Fan, Zhipeng
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background Information.
NF-κB signaling pathway plays a complicated role in the biological functions of mesenchymal stem cells.
However, the effects of NF-κB pathway on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) remain unclear.
The present study was designed to evaluate the effects of canonical NF-κB pathway on the osteo/odontogenic capacity of SCAPs in vitro.
Results.
Western blot results demonstrated that NF-κB pathway in SCAPs was successfully activated by TNF-α or blocked by BMS-345541.
NF-κB pathway-activated SCAPs presented a higher proliferation activity compared with control groups, as indicated by dimethyl-thiazol-diphenyl tetrazolium bromide assay (MTT) and flow cytometry assay (FCM).
Wound scratch assay revealed that NF-κB pathway-activated SCAPs presented an improved migration capacity, enhanced alkaline phosphatase (ALP) activity, and upregulated mineralization capacity of SCAPs, as compared with control groups.
Meanwhile, the odonto/osteogenic markers (ALP/ALP, RUNX2/RUNX2, OSX/OSX, OCN/OCN, OPN/OPN, BSP/BSP, DSPP/DSP, and DMP-1/DMP-1) in NF-κB pathway-activated SCAPs were also significantly upregulated as compared with control groups at both protein and mRNA levels.
However, NF-κB pathway-inhibited SCAPs exhibited a lower proliferation/migration capacity, and decreased odonto/osteogenic ability in comparison with control groups.
Conclusion.
Our findings suggest that classical NF-κB pathway plays a paramount role in the proliferation and committed differentiation of SCAPs.
American Psychological Association (APA)
Li, Junjun& Yan, Ming& Wang, Zilu& Jing, Shuanglin& Li, Yao& Liu, Genxia…[et al.]. 2014. Effects of Canonical NF-κB Signaling Pathway on the Proliferation and OdontoOsteogenic Differentiation of Human Stem Cells from Apical Papilla. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-463204
Modern Language Association (MLA)
Li, Junjun…[et al.]. Effects of Canonical NF-κB Signaling Pathway on the Proliferation and OdontoOsteogenic Differentiation of Human Stem Cells from Apical Papilla. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-463204
American Medical Association (AMA)
Li, Junjun& Yan, Ming& Wang, Zilu& Jing, Shuanglin& Li, Yao& Liu, Genxia…[et al.]. Effects of Canonical NF-κB Signaling Pathway on the Proliferation and OdontoOsteogenic Differentiation of Human Stem Cells from Apical Papilla. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-463204
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463204