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Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells
Joint Authors
Zhang, Yaozhou
Yao, Yuhua
Quan, Yanping
Li, Qing
Yu, Wei
Source
International Journal of Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The 30K family of proteins is important in energy metabolism and may play a role in inhibiting cellular apoptosis in silkworms (Bombyx mori).
Several 30K-family proteins have been identified.
In this study, two new silkworm genes, referred to as Slp (NM 001126256) and Lsp-t (NM 001043443), were analyzed by a bioinformatics approach according to the sequences of 30K proteins previously reported in the silkworm.
Both Slp and Lsp-t shared more than 41% amino acid sequence homology with the reported 30K proteins and displayed a conserved domain consistent with that of lipoprotein-11.
Additionally, the cDNA sequences of both Slp and Lsp-t were obtained from the fat bodies of silkworm larvae by reverse transcription polymerase chain reaction.
Both genes were expressed in BmN cells using the Bac-to-Bac system.
Purified Slp and Lsp-t were added to cultured BmN and human umbilical vein endothelial cells (HUVEC) that were treated with H2O2.
Both Slp and Lsp-t significantly enhanced the viability and suppressed DNA fragmentation in H2O2 treated BmN and HUVEC cells.
This study suggested that Slp and Lsp-t exhibit similar biological activities as their known 30K-protein counterparts and mediate an inhibitory effect against H2O2-induced apoptosis.
American Psychological Association (APA)
Yu, Wei& Li, Qing& Yao, Yuhua& Quan, Yanping& Zhang, Yaozhou. 2013. Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-463455
Modern Language Association (MLA)
Yu, Wei…[et al.]. Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells. International Journal of Genomics No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-463455
American Medical Association (AMA)
Yu, Wei& Li, Qing& Yao, Yuhua& Quan, Yanping& Zhang, Yaozhou. Two Novel 30K Proteins Overexpressed in Baculovirus System and Their Antiapoptotic Effect in Insect and Mammalian Cells. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-463455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463455