Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Joint Authors
Murata, Hiroshi
Okada, Tatsumi
Watanabe, Nobuo
Konishi, Kanako
Shirai, Kyo
Sugawara, Fumio
Arai, Takao
Akashi, Soichiro
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-09
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
3-Morpholinosydnonimine (SIN-1) is used as a donor of peroxynitrite (ONOO−) in various studies.
We demonstrated, however, that, the cell-culture medium remains cytotoxic to PC12 cells even after almost complete SIN-1 decomposition, suggesting that reaction product(s) in the medium, rather than ONOO−, exert cytotoxic effects.
Here, we clarified that significant cytotoxicity persists after SIN-1 decomposes in bicarbonate, a component of the culture medium, but not in NaOH.
Cytotoxic SIN-1-decomposed bicarbonate, which lacks both oxidizing and nitrosating activities, degrades to innocuous state over time.
The extent of SIN-1 cytotoxicity, irrespective of its fresh or decomposed state, appears to depend on the total number of initial SIN-1 molecules per cell, rather than its concentration, and involves oxidative/nitrosative stress-related cell damage.
These results suggest that, despite its low abundance, the bicarbonate-dependent cytotoxic substance that accumulates in the medium during SIN-1 breakdown is the cytotoxic entity of SIN-1.
American Psychological Association (APA)
Shirai, Kyo& Okada, Tatsumi& Konishi, Kanako& Murata, Hiroshi& Akashi, Soichiro& Sugawara, Fumio…[et al.]. 2012. Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-463760
Modern Language Association (MLA)
Shirai, Kyo…[et al.]. Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-463760
American Medical Association (AMA)
Shirai, Kyo& Okada, Tatsumi& Konishi, Kanako& Murata, Hiroshi& Akashi, Soichiro& Sugawara, Fumio…[et al.]. Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-463760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463760