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Osteoponin Promoter Controlled by DNA Methylation : Aberrant Methylation in Cloned Porcine Genome
Joint Authors
Wu, Shinn-Chih
Chen, Calvin Yu-Chian
Cheng, Winston T. K.
Chen, Chuan-Mu
Huang, Hung-Jin
Tsou, Yung-An
Shen, Chih-Jie
Chen, Hsiao-Ling
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-02
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Cloned animals usually exhibited many defects in physical characteristics or aberrant epigenetic reprogramming, especially in some important organ development.
Osteoponin (OPN) is an extracellular-matrix protein involved in heart and bone development and diseases.
In this study, we investigated the correlation between OPN mRNA and its promoter methylation changes by the 5-aza-dc treatment in fibroblast cell and promoter assay.
Aberrant methylation of porcine OPN was frequently found in different tissues of somatic nuclear transferred cloning pigs, and bisulfite sequence data suggested that the OPN promoter region −2615 to −2239 nucleotides (nt) may be a crucial regulation DNA element.
In pig ear fibroblast cell culture study, the demethylation of OPN promoter was found in dose-dependent response of 5-aza-dc treatment and followed the OPN mRNA reexpression.
In cloned pig study, discrepant expression pattern was identified in several cloned pig tissues, especially in brain, heart, and ear.
Promoter assay data revealed that four methylated CpG sites presenting in the −2615 to −2239 nt region cause significant downregulation of OPN promoter activity.
These data suggested that methylation in the OPN promoter plays a crucial role in the regulation of OPN expression that we found in cloned pigs genome.
American Psychological Association (APA)
Shen, Chih-Jie& Tsou, Yung-An& Chen, Hsiao-Ling& Huang, Hung-Jin& Wu, Shinn-Chih& Cheng, Winston T. K.…[et al.]. 2014. Osteoponin Promoter Controlled by DNA Methylation : Aberrant Methylation in Cloned Porcine Genome. BioMed Research International،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-463849
Modern Language Association (MLA)
Shen, Chih-Jie…[et al.]. Osteoponin Promoter Controlled by DNA Methylation : Aberrant Methylation in Cloned Porcine Genome. BioMed Research International No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-463849
American Medical Association (AMA)
Shen, Chih-Jie& Tsou, Yung-An& Chen, Hsiao-Ling& Huang, Hung-Jin& Wu, Shinn-Chih& Cheng, Winston T. K.…[et al.]. Osteoponin Promoter Controlled by DNA Methylation : Aberrant Methylation in Cloned Porcine Genome. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-463849
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463849