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Detection of Quadruplex DNA by Gold Nanoparticles
Joint Authors
Doudt, Alex
Crouse, Heather F.
Zerbe, Cassie
Basu, Swarna
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Gold nanoparticles have been used as a probe to detect low (<10 ppb) concentrations of quadruplex DNA.
These nanoparticles display a tendency to form aggregates in the presence of certain quadruplex forms, as observed via enhanced plasmon resonance light scattering (PRLS) signals.
These nanoparticles showed differing degrees of interactions with different types of quadruplex and mixed sequences but no interaction with duplex DNA.
Enhancement of PRLS signals greater than 50% was observed at nanomolar DNA concentration, and a lower limit of detection of 2.1 nM was established for three different quadruplex DNA sequences, including the thrombin-inhibiting single-stranded 15 mer aptamer DNA, d(GGTTGGTGTGGTTGG), and the double-stranded 12 mer DNA, d(G4T4G4).
Two different sample preparation protocols were used for the PRLS experiments, and they yielded similar results.
American Psychological Association (APA)
Crouse, Heather F.& Doudt, Alex& Zerbe, Cassie& Basu, Swarna. 2012. Detection of Quadruplex DNA by Gold Nanoparticles. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-463858
Modern Language Association (MLA)
Crouse, Heather F.…[et al.]. Detection of Quadruplex DNA by Gold Nanoparticles. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-463858
American Medical Association (AMA)
Crouse, Heather F.& Doudt, Alex& Zerbe, Cassie& Basu, Swarna. Detection of Quadruplex DNA by Gold Nanoparticles. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-463858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463858