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The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri
Joint Authors
Streif, Judith
Haufschildt, Kristin
Storbeck, Sonja
Kühner, Melanie
Layer, Gunhild
Neumann, Alexander
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-23
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In living organisms heme is formed from the common precursor uroporphyrinogen III by either one of two substantially different pathways.
In contrast to eukaryotes and most bacteria which employ the so-called “classical” heme biosynthesis pathway, the archaea use an alternative route.
In this pathway, heme is formed from uroporphyrinogen III via the intermediates precorrin-2, sirohydrochlorin, siroheme, 12,18-didecarboxysiroheme, and iron-coproporphyrin III.
In this study the heme biosynthesis proteins AhbAB, AhbC, and AhbD from Methanosarcina barkeri were functionally characterized.
Using an in vivo enzyme activity assay it was shown that AhbA and AhbB (Mbar_A1459 and Mbar_A1460) together catalyze the conversion of siroheme into 12,18-didecarboxysiroheme.
The two proteins form a heterodimeric complex which might be subject to feedback regulation by the pathway end-product heme.
Further, AhbC (Mbar_A1793) was shown to catalyze the formation of iron-coproporphyrin III in vivo.
Finally, recombinant AhbD (Mbar_A1458) was produced in E.
coli and purified indicating that this protein most likely contains two [4Fe-4S] clusters.
Using an in vitro enzyme activity assay it was demonstrated that AhbD catalyzes the conversion of iron-coproporphyrin III into heme.
American Psychological Association (APA)
Kühner, Melanie& Haufschildt, Kristin& Neumann, Alexander& Storbeck, Sonja& Streif, Judith& Layer, Gunhild. 2014. The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri. Archaea،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-463863
Modern Language Association (MLA)
Kühner, Melanie…[et al.]. The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri. Archaea No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-463863
American Medical Association (AMA)
Kühner, Melanie& Haufschildt, Kristin& Neumann, Alexander& Storbeck, Sonja& Streif, Judith& Layer, Gunhild. The Alternative Route to Heme in the Methanogenic Archaeon Methanosarcina barkeri. Archaea. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-463863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463863