![](/images/graphics-bg.png)
Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology
Joint Authors
Vainstein, Marilene Henning
Bussamara, Roberta
Dall'Agnol, Luciane
Schrank, Augusto
Fernandes, Kátia Flávia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production.
An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support.
Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme.
The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support.
Optimal activity temperature for free and immobilized enzyme was found to be 68°C and 52°C, respectively.
Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively.
Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone.
American Psychological Association (APA)
Bussamara, Roberta& Dall'Agnol, Luciane& Schrank, Augusto& Fernandes, Kátia Flávia& Vainstein, Marilene Henning. 2012. Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology. Enzyme Research،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-463990
Modern Language Association (MLA)
Bussamara, Roberta…[et al.]. Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology. Enzyme Research No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-463990
American Medical Association (AMA)
Bussamara, Roberta& Dall'Agnol, Luciane& Schrank, Augusto& Fernandes, Kátia Flávia& Vainstein, Marilene Henning. Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-463990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-463990