DNA Barcoding and Molecular Phylogeny of Drosophila lini and Its Sibling Species
Joint Authors
Segawa, Ryoko
Gao, Jian-Jun
Toda, Masanori J.
Hu, Yao-Guang
Wen, Shuo-Yang
Li, Yi-Feng
Kawai, Kuniko
Source
International Journal of Evolutionary Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Drosophila lini and its two sibling species, D.
ohnishii and D.
ogumai, are hardly distinguishable from one another in morphology.
These species are more or less reproductively isolated.
The mitochondrial ND2 and COI-COII and the nuclear ITS1-ITS2 regions were sequenced to seek for the possibility of DNA barcoding and to reconstruct the phylogeny of them.
The character-based approach for DNA barcoding detected some diagnostic nucleotides only for monophyletic D.
ogumai, but no informative sites for the other two very closely species, D.
lini and D.
ohnishii, of which strains intermingled in the molecular phylogenetic trees.
Thus, this study provides another case of limited applicability of DNA barcoding in species delineation, as in other cases of related Drosophila species.
The molecular phylogenetic tree inferred from the concatenated sequences strongly supported the monophyly of the cluster of the three species, that is, the lini clade.
We propose some hypotheses of evolutionary events in this clade.
American Psychological Association (APA)
Li, Yi-Feng& Wen, Shuo-Yang& Kawai, Kuniko& Gao, Jian-Jun& Hu, Yao-Guang& Segawa, Ryoko…[et al.]. 2012. DNA Barcoding and Molecular Phylogeny of Drosophila lini and Its Sibling Species. International Journal of Evolutionary Biology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-464021
Modern Language Association (MLA)
Li, Yi-Feng…[et al.]. DNA Barcoding and Molecular Phylogeny of Drosophila lini and Its Sibling Species. International Journal of Evolutionary Biology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-464021
American Medical Association (AMA)
Li, Yi-Feng& Wen, Shuo-Yang& Kawai, Kuniko& Gao, Jian-Jun& Hu, Yao-Guang& Segawa, Ryoko…[et al.]. DNA Barcoding and Molecular Phylogeny of Drosophila lini and Its Sibling Species. International Journal of Evolutionary Biology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-464021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464021