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Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics
Joint Authors
Chan, C. Y. X'avia
Siu, K. W. Michael
McDermott, John C.
Source
International Journal of Proteomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Myogenesis, the formation of skeletal muscle, is a multistep event that commences with myoblast proliferation, followed by cell-cycle arrest, and finally the formation of multinucleated myotubes via fusion of mononucleated myoblasts.
Each step is orchestrated by well-documented intracellular factors, such as cytoplasmic signalling molecules and nuclear transcription factors.
Regardless, the key step in getting a more comprehensive understanding of the regulation of myogenesis is to explore the extracellular factors that are capable of eliciting the downstream intracellular factors.
This could further provide valuable insight into the acute cellular response to extrinsic cues in maintaining normal muscle development.
In this paper, we survey the intracellular factors that respond to extracellular cues that are responsible for the cascades of events during myogenesis: myoblast proliferation, cell-cycle arrest of myoblasts, and differentiation of myoblasts into myotubes.
This focus on extracellular perspective of muscle development illustrates our mass spectrometry-based proteomic approaches to identify differentially expressed secreted factors during skeletal myogenesis.
American Psychological Association (APA)
Chan, C. Y. X'avia& McDermott, John C.& Siu, K. W. Michael. 2011. Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-464027
Modern Language Association (MLA)
Chan, C. Y. X'avia…[et al.]. Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics. International Journal of Proteomics No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-464027
American Medical Association (AMA)
Chan, C. Y. X'avia& McDermott, John C.& Siu, K. W. Michael. Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-464027
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464027