Prostaglandin D2 in Inflammatory Arthritis and Its Relation with Synovial Fluid Dendritic Cells
Joint Authors
Moghaddami, Mahin
Ranieri, Enzo
Cleland, Leslie G.
Fletcher, Janice
James, Michael
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Prostaglandin (PG)D2 has been shown to be an active agent in the resolution of experimentally induced inflammation.
This study was undertaken to determine the presence of PGD2 in chronic joint effusions and to explore the potential contributions of dendritic cells (DC) and monocytes to the intra-articular synthesis of PGD2.
Synovial fluid (SF) was obtained from patients with inflammatory arthritis and knee effusions.
PGD2 and PGE2 were detected in SF by ultrahigh-performance tandem mass spectrometry.
Cellular fractions in SF were separated by density-gradient centrifugation and flow cytometry.
The expression of hematopoietic prostaglandin D-synthase (hPGDS) and PGE-synthase (PGES) mRNA was determined by RT-PCR.
Both PGD2 and PGE2 were detected in blood and SF, with PGD2 being more abundant than PGE2 in SF.
mRNA for hPGDS was more abundant in SF mDCs than SF monocytes (P<0.01) or PB monocytes (P<0.001).
SF mDC expressed significantly more hPGDS than PGES.
Expressions of PGD2 and hPGDS were inversely associated with serum C-reactive protein (P<0.01) and erythrocyte sedimentation rate (P<0.01).
The findings suggest that synovial DCs may be an important source of hPGDS and that systemic disease activity may be influenced by actions of PGD2 in RA and other arthropathies.
American Psychological Association (APA)
Moghaddami, Mahin& Ranieri, Enzo& James, Michael& Fletcher, Janice& Cleland, Leslie G.. 2013. Prostaglandin D2 in Inflammatory Arthritis and Its Relation with Synovial Fluid Dendritic Cells. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-464032
Modern Language Association (MLA)
Moghaddami, Mahin…[et al.]. Prostaglandin D2 in Inflammatory Arthritis and Its Relation with Synovial Fluid Dendritic Cells. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-464032
American Medical Association (AMA)
Moghaddami, Mahin& Ranieri, Enzo& James, Michael& Fletcher, Janice& Cleland, Leslie G.. Prostaglandin D2 in Inflammatory Arthritis and Its Relation with Synovial Fluid Dendritic Cells. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-464032
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464032