Evaluation of Hepcidin Isoforms in Hemodialysis Patients by a Proteomic Approach Based on SELDI-TOF MS
Joint Authors
Olivieri, Oliviero
Martinelli, Nicola
Campostrini, Natascia
Zaninotto, Federica
Poli, Albino
Tessitore, Nicola
Bedogna, Valeria
Girelli, Domenico
Lupo, Antonio
Castagna, Annalisa
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The hepatic iron regulator hormone hepcidin consists, in its mature form, of 25 amino acids, but two other isoforms, hepcidin-20 and hepcidin-22, have been reported, whose biological meaning remains poorly understood.
We evaluated hepcidin isoforms in sera from 57 control and 54 chronic haemodialysis patients using a quantitative proteomic approach based on SELDI-TOF-MS.
Patients had elevated serum levels of both hepcidin-25 and hepcidin-20 as compared to controls (geometric means: 7.52 versus 4.69 nM, and 4.06 versus 1.76 nM, resp., P<.05 for both).
The clearance effects of a single dialysis session by different dialysis techniques and membranes were also investigated, showing an average reduction by 51.3% ± 29.2% for hepcidin-25 and 34.2% ± 28.4% for hepcidin-20 but only minor differences among the different dialysis modalities.
Measurement of hepcidin isoforms through MS-based techniques can be a useful tool for better understanding of their biological role in hemodialysis patients and other clinical conditions.
American Psychological Association (APA)
Campostrini, Natascia& Castagna, Annalisa& Zaninotto, Federica& Bedogna, Valeria& Tessitore, Nicola& Poli, Albino…[et al.]. 2010. Evaluation of Hepcidin Isoforms in Hemodialysis Patients by a Proteomic Approach Based on SELDI-TOF MS. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-464058
Modern Language Association (MLA)
Campostrini, Natascia…[et al.]. Evaluation of Hepcidin Isoforms in Hemodialysis Patients by a Proteomic Approach Based on SELDI-TOF MS. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-464058
American Medical Association (AMA)
Campostrini, Natascia& Castagna, Annalisa& Zaninotto, Federica& Bedogna, Valeria& Tessitore, Nicola& Poli, Albino…[et al.]. Evaluation of Hepcidin Isoforms in Hemodialysis Patients by a Proteomic Approach Based on SELDI-TOF MS. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-464058
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464058