Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer : Evidences for a Direct GRP-Mineral Interaction
Joint Authors
Cavaco, Sofia I.
Teixeira, Alexandra
Ferreira, Ana
Enriquez, José L.
Theuwissen, Elke
João, Alexandre
van ‘t Hoofd, Cynthia M. R.
Serra, Manuel
Rafael, Marta S.
Maria, Vera L.
Luís, Inês M.
Viegas, Carla S. B.
Herfs, Marjolein
Simes, Dina C.
Vermeer, Cees
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Gla-rich protein (GRP) was described in sturgeon as a new vitamin-K-dependent protein (VKDP) with a high density of Gla residues and associated with ectopic calcifications in humans.
Although VKDPs function has been related with γ-carboxylation, the Gla status of GRP in humans is still unknown.
Here, we investigated the expression of recently identified GRP spliced transcripts, the γ-carboxylation status, and its association with ectopic calcifications, in skin basal cell and breast carcinomas.
GRP-F1 was identified as the predominant splice variant expressed in healthy and cancer tissues.
Patterns of γ-carboxylated GRP (cGRP)/undercarboxylated GRP (ucGRP) accumulation in healthy and cancer tissues were determined by immunohistochemistry, using newly developed conformation-specific antibodies.
Both GRP protein forms were found colocalized in healthy tissues, while ucGRP was the predominant form associated with tumor cells.
Both cGRP and ucGRP found at sites of microcalcifications were shown to have in vitro calcium mineral-binding capacity.
The decreased levels of cGRP and predominance of ucGRP in tumor cells suggest that GRP may represent a new target for the anticancer potential of vitamin K.
Also, the direct interaction of cGRP and ucGRP with BCP crystals provides a possible mechanism explaining GRP association with pathological mineralization.
American Psychological Association (APA)
Viegas, Carla S. B.& Herfs, Marjolein& Rafael, Marta S.& Enriquez, José L.& Teixeira, Alexandra& Luís, Inês M.…[et al.]. 2014. Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer : Evidences for a Direct GRP-Mineral Interaction. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-464097
Modern Language Association (MLA)
Viegas, Carla S. B.…[et al.]. Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer : Evidences for a Direct GRP-Mineral Interaction. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-464097
American Medical Association (AMA)
Viegas, Carla S. B.& Herfs, Marjolein& Rafael, Marta S.& Enriquez, José L.& Teixeira, Alexandra& Luís, Inês M.…[et al.]. Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer : Evidences for a Direct GRP-Mineral Interaction. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-464097
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464097