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Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum
Joint Authors
Mutreja, Ruchi
Das, Debasish
Goyal, Dinesh
Goyal, Arun
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The effect of different pretreatment methods, temperature, and enzyme concentration on ethanol production from 8 lignocellulosic agrowaste by simultaneous saccharification and fermentation (SSF) using recombinant cellulase and Saccharomyces cerevisiae were studied.
Recombinant cellulase was isolated from E.
coli BL21 cells transformed with CtLic26A-Cel5-CBM11 full-length gene from Clostridium thermocellum and produced in both batch and fed-batch processes.
The maximum cell OD and specific activity in batch mode were 1.6 and 1.91 U/mg, respectively, whereas in the fed-batch mode, maximum cell OD and specific activity were 3.8 and 3.5 U/mg, respectively, displaying a 2-fold increase.
Eight substrates, Syzygium cumini (jamun), Azadirachta indica (neem), Saracens indica (asoka), bambusa dendrocalmus (bamboo), Populas nigra (poplar), Achnatherum hymenoides (wild grass), Eucalyptus marginata (eucalyptus), and Mangifera indica (mango), were subjected to SSF.
Of three pretreatments, acid, alkali, and steam explosion, acid pretreatment Syzygium cumini (Jamun) at 30°C gave maximum ethanol yield of 1.42 g/L.
American Psychological Association (APA)
Mutreja, Ruchi& Das, Debasish& Goyal, Dinesh& Goyal, Arun. 2011. Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum. Enzyme Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-464106
Modern Language Association (MLA)
Mutreja, Ruchi…[et al.]. Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum. Enzyme Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-464106
American Medical Association (AMA)
Mutreja, Ruchi& Das, Debasish& Goyal, Dinesh& Goyal, Arun. Bioconversion of Agricultural Waste to Ethanol by SSF Using Recombinant Cellulase from Clostridium thermocellum. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-464106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464106