Hepatic Gene Expression Profiling in Nrf2 Knockout Mice after Long-Term High-Fat Diet-Induced Obesity
Joint Authors
Ziros, Panos G.
Zaravinos, Apostolos
Sykiotis, Gerasimos P.
Kyriazopoulou, Venetsana E.
Iskrenova, Ralitsa P.
Chartoumpekis, Dionysios V.
Psyrogiannis, Agathoklis I.
Habeos, Ioannis G.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-18
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Introduction.
The transcription factor NFE2-related factor 2 (Nrf2) is a central regulator of antioxidant and detoxification gene expression in response to electrophilic or oxidative stress.
Nrf2 has recently been shown to cross-talk with metabolic pathways, and its gene deletion protected mice from high-fat-diet-(HFD-) induced obesity and insulin resistance.
This study aimed to identify potential Nrf2-regulated genes of metabolic interest by comparing gene expression profiles of livers of wild-type (WT) versus Nrf2 knockout (Nrf2-KO) mice after a long-term HFD.
Methods.
WT and Nrf2-KO mice were fed an HFD for 180 days; total RNA was prepared from liver and used for microarray analysis and quantitative real-time RT-PCR (qRT-PCR).
Results.
The microarray analysis identified 601 genes that were differentially expressed between WT and Nrf2-KO mice after long-term HFD.
Selected genes, including ones known to be involved in metabolic regulation, were prioritized for verification by qRT-PCR: Cyp7a1 and Fabp5 were significantly overexpressed in Nrf2-KO mice; in contrast, Car, Cyp2b10, Lipocalin 13, Aquaporin 8, Cbr3, Me1, and Nqo1 were significantly underexpressed in Nrf2-KO mice.
Conclusion.
Transcriptome profiling after HFD-induced obesity confirms that Nrf2 is implicated in liver metabolic gene networks.
The specific genes identified here may provide insights into Nrf2-dependent mechanisms of metabolic regulation.
American Psychological Association (APA)
Chartoumpekis, Dionysios V.& Ziros, Panos G.& Zaravinos, Apostolos& Iskrenova, Ralitsa P.& Psyrogiannis, Agathoklis I.& Kyriazopoulou, Venetsana E.…[et al.]. 2013. Hepatic Gene Expression Profiling in Nrf2 Knockout Mice after Long-Term High-Fat Diet-Induced Obesity. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-464161
Modern Language Association (MLA)
Chartoumpekis, Dionysios V.…[et al.]. Hepatic Gene Expression Profiling in Nrf2 Knockout Mice after Long-Term High-Fat Diet-Induced Obesity. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-17.
https://search.emarefa.net/detail/BIM-464161
American Medical Association (AMA)
Chartoumpekis, Dionysios V.& Ziros, Panos G.& Zaravinos, Apostolos& Iskrenova, Ralitsa P.& Psyrogiannis, Agathoklis I.& Kyriazopoulou, Venetsana E.…[et al.]. Hepatic Gene Expression Profiling in Nrf2 Knockout Mice after Long-Term High-Fat Diet-Induced Obesity. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-464161
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464161