Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography
Joint Authors
Tang, Cha-Mei
Adams, Daniel L.
Stefansson, Steingrimur
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Conventional protein affinity chromatography relies on highly porous resins that have large surface areas.
These properties are ideal for fast flow separation of proteins from biological samples with maximum yields, but these properties can also lead to increased nonspecific protein binding.
In certain applications where the purity of an isolated protein is more important than the yield, using a glass solid phase could be advantageous as glass is nonporous and hydrophilic and has a low surface area and low nonspecific protein binding.
As a proof of principle, we used protein A-conjugated hollow glass microbubbles to isolate fluorescently labeled neurofilament heavy chain spiked into serum and compared them to protein A Sepharose and protein A magnetic beads (Dynabeads) using an anti-neurofilament protein antibody.
As expected, a greater volume of glass bubbles was required to match the binding capacity of the magnetic beads and Sepharose resins.
On the other hand, nonspecific protein binding to glass bubbles was greatly reduced compared to the other resins.
Additionally, since the glass bubbles are buoyant and transparent, they are well suited for isolating cells from biological samples and staining them in situ.
American Psychological Association (APA)
Stefansson, Steingrimur& Adams, Daniel L.& Tang, Cha-Mei. 2013. Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography. Chromatography Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-464201
Modern Language Association (MLA)
Stefansson, Steingrimur…[et al.]. Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography. Chromatography Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-464201
American Medical Association (AMA)
Stefansson, Steingrimur& Adams, Daniel L.& Tang, Cha-Mei. Isolation of Low Abundance Proteins and Cells Using Buoyant Glass Microbubble Chromatography. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-464201
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464201