Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride
Joint Authors
Prasad, K. Nagendra
Yu, Wenlan
Li, Ying
Hu, Lianmei
Tang, Zhaoxin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The antioxidant activities and protective effects of total phenolic extracts (TPE) and their major components from okra seeds on oxidative stress induced by carbon tetrachloride (CCl4) in rat hepatocyte cell line were investigated.
The major phenolic compounds were identified as quercetin 3-O-glucosyl (1→6) glucoside (QDG) and quercetin 3-O-glucoside (QG).
TPE, QG, and QDG from okra seeds exhibited excellent reducing power and free radical scavenging capabilities including α, α-diphenyl-β-picrylhydrazyl (DPPH), superoxide anions, and hydroxyl radical.
Overall, DPPH radical scavenging activity and reducing power of QG and QDG were higher than those of TPE while superoxide and hydroxyl radical scavenging activities of QG and TPE were higher than those of QDG.
Furthermore, TPE, QG, and QDG pretreatments significantly alleviated the cytotoxicity of CCl4 on rat hepatocytes, with attenuated lipid peroxidation, increased SOD and CAT activities, and decreased GPT and GOT activities.
The protective effects of TPE and QG on rat hepatocytes were stronger than those of QDG.
However, the cytotoxicity of CCl4 on rat hepatocytes was not affected by TPE, QG, and QDG posttreatments.
It was suggested that the protective effects of TPE, QG, and QDG on rat hepatocyte against oxidative stress were related to the direct antioxidant capabilities and the induced antioxidant enzymes activities.
American Psychological Association (APA)
Hu, Lianmei& Yu, Wenlan& Li, Ying& Prasad, K. Nagendra& Tang, Zhaoxin. 2014. Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-464216
Modern Language Association (MLA)
Hu, Lianmei…[et al.]. Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-464216
American Medical Association (AMA)
Hu, Lianmei& Yu, Wenlan& Li, Ying& Prasad, K. Nagendra& Tang, Zhaoxin. Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-464216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464216