Long-Term Survival of Mycobacterium avium subsp. paratuberculosis in Fecal Samples Obtained from Naturally Infected Cows and Stored at −18°C and −70°C
Joint Authors
Espejo, L. A.
Raizman, Eran A.
Wells, S. J.
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The objective was to evaluate the survival of Mycobacterium paratuberculosis (Map) in naturally infected dairy cows feces under long periods of freezing at −18°C and −70°C.
Samples were collected from cows previously tested positive with serum ELISA or fecal culture, or with clinical signs of Johne's disease.
Samples were stored at −18°C and/or −70°C and recultured in Herrold's egg yolk media every 3–6 months.
A proportional odds mixed model was used for data analysis.
Sixty nine fecal samples were stored for different periods between September 2002 and January 2005.
Of these, 45 (65%) were stored at −18°C and 24 (35%) at −70°C.
Average number of days between repeated culture dates was 98 and 84 for −18°C and −70°C, respectively.
Median number of repeated cultures was 6 and 4 for samples stored at −18°C and −70°C, respectively.
After adjusting for initial sample bacterial load, the effects of temperature or number of thawing and refreezing cycles on Map viability were not significant.
The probability that a sample decreases from high to moderate-low bacterial load and from moderate-low to negative bacterial load was 13.5% per month.
Although this study found gradual reduction of Map concentration in stored fecal samples through time, overall survival in −18°C can ease fecal samples management in laboratories with low-processing capacity or lack of −70°C freezer.
American Psychological Association (APA)
Raizman, Eran A.& Espejo, L. A.& Wells, S. J.. 2011. Long-Term Survival of Mycobacterium avium subsp. paratuberculosis in Fecal Samples Obtained from Naturally Infected Cows and Stored at −18°C and −70°C. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-464261
Modern Language Association (MLA)
Raizman, Eran A.…[et al.]. Long-Term Survival of Mycobacterium avium subsp. paratuberculosis in Fecal Samples Obtained from Naturally Infected Cows and Stored at −18°C and −70°C. Veterinary Medicine International No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-464261
American Medical Association (AMA)
Raizman, Eran A.& Espejo, L. A.& Wells, S. J.. Long-Term Survival of Mycobacterium avium subsp. paratuberculosis in Fecal Samples Obtained from Naturally Infected Cows and Stored at −18°C and −70°C. Veterinary Medicine International. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-464261
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464261