Human Myocardial Protein Pattern Reveals Cardiac Diseases
Joint Authors
Baykut, Doan
Bergquist, Maria
Mayer, Franz-Josef
Baykut, Gökhan
Witt, Matthias
Bergquist, Jonas
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-08
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Proteomic profiles of myocardial tissue in two different etiologies of heart failure were investigated using high performance liquid chromatography (HPLC)/Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).
Right atrial appendages from 10 patients with hemodynamically significant isolated aortic valve disease and from 10 patients with isolated symptomatic coronary heart disease were collected during elective cardiac surgery.
As presented in an earlier study by our group (Baykut et al., 2006), both disease forms showed clearly different pattern distribution characteristics.
Interesting enough, the classification patterns could be used for correctly sorting unknown test samples in their correct categories.
However, in order to fully exploit and also validate these findings there is a definite need for unambiguous identification of the differences between different etiologies at molecular level.
In this study, samples representative for the aortic valve disease and coronary heart disease were prepared, tryptically digested, and analyzed using an FT-ICR MS that allowed collision-induced dissociation (CID) of selected classifier masses.
By using the fragment spectra, proteins were identified by database searches.
For comparison and further validation, classifier masses were also fragmented and analyzed using HPLC-/Matrix-assisted laser desorption ionization (MALDI) time-of-flight/time-of-flight (TOF/TOF) mass spectrometry.
Desmin and lumican precursor were examples of proteins found in aortic samples at higher abundances than in coronary samples.
Similarly, adenylate kinase isoenzyme was found in coronary samples at a higher abundance.
The described methodology could also be feasible in search for specific biomarkers in plasma or serum for diagnostic purposes.
American Psychological Association (APA)
Bergquist, Jonas& Baykut, Gökhan& Bergquist, Maria& Witt, Matthias& Mayer, Franz-Josef& Baykut, Doan. 2012. Human Myocardial Protein Pattern Reveals Cardiac Diseases. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-464341
Modern Language Association (MLA)
Bergquist, Jonas…[et al.]. Human Myocardial Protein Pattern Reveals Cardiac Diseases. International Journal of Proteomics No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-464341
American Medical Association (AMA)
Bergquist, Jonas& Baykut, Gökhan& Bergquist, Maria& Witt, Matthias& Mayer, Franz-Josef& Baykut, Doan. Human Myocardial Protein Pattern Reveals Cardiac Diseases. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-464341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464341