![](/images/graphics-bg.png)
Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania
Joint Authors
Matee, Mecky
von Reyn, C. Fordham
Adams, Lisa V.
Ferguson, Katherine
Talbot, Elizabeth A.
Maro, Isaac
Mtei, Lillian
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-06
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Background.
T-SPOT.TB is an interferon gamma release assay for detecting Mycobacterium tuberculosis infection.
The requirement to process within 8 hours is constraining, deters use, and leads to invalid results.
Addition of T Cell Xtend reagent may allow delayed processing, but has not been extensively field tested.
Design.
Consecutive AFB smear positive adult tuberculosis patients were prospectively recruited in Dar es Salaam, Tanzania.
Patients provided a medical history, 1–3 sputum samples for culture and 1 blood sample which was transported to the laboratory under temperature-controlled conditions.
After overnight storage, 25 μL of T Cell Xtend reagent was added per mL of blood, and the sample was tested using T-SPOT.TB.
Results.
143 patients were enrolled: 57 patients were excluded because temperature control was not maintained, 19 patients were excluded due to red blood cell contamination, and one did not provide a sputum sample for culture.
Among 66 evaluable patients, overall agreement between T-SPOT.TB and culture was 95.4% (95%CI; 87.1–99.0%) with Kappa value 0.548.
Sensitivity of T-SPOT.TB when using T Cell Xtend reagent was 96.8% (95%CI; 88.8–99.6%).
Conclusions.
When T Cell Xtend reagent is added to specimens held overnight at recommended temperatures, T-SPOT.TB is as sensitive as the standard assay in patients with tuberculosis.
American Psychological Association (APA)
Talbot, Elizabeth A.& Maro, Isaac& Ferguson, Katherine& Adams, Lisa V.& Mtei, Lillian& Matee, Mecky…[et al.]. 2012. Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-464466
Modern Language Association (MLA)
Talbot, Elizabeth A.…[et al.]. Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-464466
American Medical Association (AMA)
Talbot, Elizabeth A.& Maro, Isaac& Ferguson, Katherine& Adams, Lisa V.& Mtei, Lillian& Matee, Mecky…[et al.]. Maintenance of Sensitivity of the T-SPOT.TB Assay after Overnight Storage of Blood Samples, Dar es Salaam, Tanzania. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-464466
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464466