![](/images/graphics-bg.png)
An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium (L) Schott]
Joint Authors
Poor, Abbas Tahmasebi
Sama, Anne E.
Hughes, Harrison G.
Shahba, Mohamed A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Sprouted corm sections of “South Dade” white cocoyam were potted and maintained in a greenhouse for 8 weeks.
Shoot tips of 3–5 mm comprising the apical meristem with 4–6 leaf primordial, and approximately 0.5 mm of corm tissue at the base.
These explants were treated to be used into the culture medium.
A modified Gamborg’s B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA) were used throughout the study.
Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used.
Erlenmeyer flasks and test tubes were used for growing cultures.
The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested.
Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures.
All concentrations tested (5–20 μM BAP and 1–4 μM TDZ) produced more axillary shoots per shoot tip than the control without cytokinins.
Greater proliferation rates were obtained through the use of 20 μM BAP and 2 μM TDZ, respectively, 12 weeks from initiation.
Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted.
The use of stationary liquid media is recommended for economic reasons.
American Psychological Association (APA)
Sama, Anne E.& Hughes, Harrison G.& Poor, Abbas Tahmasebi& Shahba, Mohamed A.. 2012. An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium (L) Schott]. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-464578
Modern Language Association (MLA)
Sama, Anne E.…[et al.]. An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium (L) Schott]. The Scientific World Journal No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-464578
American Medical Association (AMA)
Sama, Anne E.& Hughes, Harrison G.& Poor, Abbas Tahmasebi& Shahba, Mohamed A.. An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium (L) Schott]. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-464578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464578