The Modulatory Effect of Ellagic Acid and Rosmarinic Acid on Ultraviolet-B-Induced CytokineChemokine Gene Expression in Skin Keratinocyte (HaCaT) Cells
Joint Authors
Monfrecola, Giuseppe
Gasparri, Franco
Balato, Anna
Cirillo, Teresa
Lembo, Serena
Giannini, Valentina
Di Caprio, Roberta
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Ultraviolet radiation (UV) induces an increase in multiple cutaneous inflammatory mediators.
Ellagic acid (EA) and rosmarinic acid (RA) are natural anti-inflammatory and immunomodulatory compounds found in many plants, fruits, and nuts.
We assessed the ability of EA and RA to modulate IL-1β, IL-6, IL-8, IL-10, MCP-1, and TNF-α gene expression in HaCaT cells after UVB irradiation.
Cells were treated with UVB (100 mJ/cm2) and simultaneously with EA (5 μM in 0.1% DMSO) or RA (2.7 μM in 0.5% DMSO).
Moreover, these substances were added to the UVB-irradiated cells 1 h or 6 h before harvesting, depending on the established UVB-induced cytokine expression peak.
Cytokine gene expression was examined using quantitative real time polymerase chain reaction.
RA produced a significant reduction in UVB-induced expression of IL-6, IL-8, MCP-1, and TNF-α when applied at the same time as irradiation.
EA showed milder effects compared with RA, except for TNF-α.
Both substances decreased IL-6 expression, also when applied 5 h after irradiation, and always produced a significant increase in UVB-induced IL-10 expression.
Our findings suggest that EA and RA are able to prevent and/or limit the UVB-induced inflammatory cascade, through a reduction in proinflammatory mediators and the enhancement of IL-10, with its protective function.
American Psychological Association (APA)
Lembo, Serena& Balato, Anna& Di Caprio, Roberta& Cirillo, Teresa& Giannini, Valentina& Gasparri, Franco…[et al.]. 2014. The Modulatory Effect of Ellagic Acid and Rosmarinic Acid on Ultraviolet-B-Induced CytokineChemokine Gene Expression in Skin Keratinocyte (HaCaT) Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-464590
Modern Language Association (MLA)
Lembo, Serena…[et al.]. The Modulatory Effect of Ellagic Acid and Rosmarinic Acid on Ultraviolet-B-Induced CytokineChemokine Gene Expression in Skin Keratinocyte (HaCaT) Cells. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-464590
American Medical Association (AMA)
Lembo, Serena& Balato, Anna& Di Caprio, Roberta& Cirillo, Teresa& Giannini, Valentina& Gasparri, Franco…[et al.]. The Modulatory Effect of Ellagic Acid and Rosmarinic Acid on Ultraviolet-B-Induced CytokineChemokine Gene Expression in Skin Keratinocyte (HaCaT) Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-464590
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464590