rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ
Joint Authors
Madry, Henning
Schmitt, Gertrud
Venkatesan, Jagadeesh K.
Schetting, Sarah
Cucchiarini, Magali
Kohn, Dieter
Ekici, Myriam
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Treatment of chondrosarcoma remains a major challenge in orthopaedic oncology.
Gene transfer strategies based on recombinant adenoassociated viral (rAAV) vectors may provide powerful tools to develop new, efficient therapeutic options against these tumors.
In the present study, we tested the hypothesis that rAAV is adapted for a stable and safe delivery of foreign sequences in human chondrosarcoma tissue by transducing primary human chondrosarcoma cells in vitro and in situ with different reporter genes (E.
coli lacZ, firefly luc, Discosoma sp.
RFP).
The effects of rAAV administration upon cell survival and metabolic activities were also evaluated to monitor possibly detrimental effects of the gene transfer method.
Remarkably, we provide evidence that efficient and prolonged expression of transgene sequences via rAAV can be safely achieved in all the systems investigated, demonstrating the potential of the approach of direct application of therapeutic gene vectors as a means to treat chondrosarcoma.
American Psychological Association (APA)
Madry, Henning& Venkatesan, Jagadeesh K.& Schmitt, Gertrud& Schetting, Sarah& Ekici, Myriam& Kohn, Dieter…[et al.]. 2012. rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ. Sarcoma،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-464646
Modern Language Association (MLA)
Madry, Henning…[et al.]. rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ. Sarcoma No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-464646
American Medical Association (AMA)
Madry, Henning& Venkatesan, Jagadeesh K.& Schmitt, Gertrud& Schetting, Sarah& Ekici, Myriam& Kohn, Dieter…[et al.]. rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ. Sarcoma. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-464646
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464646