Interleukin-13, but Not Indomethacin, Increases Cysteinyl-Leukotriene Synthesis in Human Lung Macrophages
Joint Authors
Sampson, Anthony P.
Warner, Jane A.
Jackson, Sarah E.
Holloway, John W.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aspirin-exacerbated respiratory disease (AERD) is associated with constitutively elevated synthesis of bronchoconstrictor cysteinyl-leukotrienes, associated with increased expression of leukotriene (LT)C4 synthase and Th2 cytokines and airway eosinophilia.
We examined whether interleukin-13 can increase LTC4 synthase gene transcription and cysteinyl-leukotriene synthesis in macrophages isolated from resected human lung tissue and whether an NSAID (indomethacin) can trigger further cysteinyl-leukotriene synthesis in these cells.
Overnight culture of human lung macrophages with IL-13 (10 ng/mL) increased spontaneous and ionophore-stimulated production of cysteinyl-leukotrienes by 42% (P=0.02) and 52% (P=0.005), respectively, as quantified by enzyme immunoassays, but PCR gene transcription assays did not demonstrate an effect on LTC4S mRNA.
The addition of indomethacin (100 μM) did not modulate cysteinyl-leukotriene production in either IL-13-treated or untreated macrophages.
We conclude that while IL-13 enhances cysteinyl-leukotriene synthesis in human lung macrophages, it does not replicate the enhanced LTC4 synthase expression observed in the AERD lung nor confer sensitivity to NSAIDs.
American Psychological Association (APA)
Jackson, Sarah E.& Holloway, John W.& Warner, Jane A.& Sampson, Anthony P.. 2011. Interleukin-13, but Not Indomethacin, Increases Cysteinyl-Leukotriene Synthesis in Human Lung Macrophages. Journal of Allergy،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-464769
Modern Language Association (MLA)
Jackson, Sarah E.…[et al.]. Interleukin-13, but Not Indomethacin, Increases Cysteinyl-Leukotriene Synthesis in Human Lung Macrophages. Journal of Allergy No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-464769
American Medical Association (AMA)
Jackson, Sarah E.& Holloway, John W.& Warner, Jane A.& Sampson, Anthony P.. Interleukin-13, but Not Indomethacin, Increases Cysteinyl-Leukotriene Synthesis in Human Lung Macrophages. Journal of Allergy. 2011. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-464769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-464769