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Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats
Joint Authors
Bennink, Maurice R.
Rondini, Elizabeth A.
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-08
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
We previously demonstrated that black bean (BB) and soy flour (SF)-based diets inhibit azoxymethane (AOM)-induced colon cancer.
The objective of this study was to identify genes altered by carcinogen treatment in normal-appearing colonic mucosa and those attenuated by bean feeding.
Ninety-five male F344 rats were fed control (AIN) diets upon arrival.
At 4 and 5 weeks, rats were injected with AOM (15 mg/kg) or saline and one week later administered an AIN, BB-, or SF-based diet.
Rats were sacrificed after 31 weeks, and microarrays were conducted on RNA isolated from the distal colonic mucosa.
AOM treatment induced a number of genes involved in immunity, including several MHC II-associated antigens and innate defense genes (RatNP-3, Lyz2, Pla2g2a).
BB- and SF-fed rats exhibited a higher expression of genes involved in energy metabolism and water and sodium absorption and lower expression of innate (RatNP-3, Pla2g2a, Tlr4, Dmbt1) and cell cycle-associated (Cdc2, Ccnb1, Top2a) genes.
Genes involved in the extracellular matrix (Col1a1, Fn1) and innate immunity (RatNP-3, Pla2g2a) were induced by AOM in all diets, but to a lower extent in bean-fed animals.
This profile suggests beans inhibit colon carcinogenesis by modulating cellular kinetics and reducing inflammation, potentially by preserving mucosal barrier function.
American Psychological Association (APA)
Rondini, Elizabeth A.& Bennink, Maurice R.. 2012. Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats. Journal of Nutrition and Metabolism،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-465056
Modern Language Association (MLA)
Rondini, Elizabeth A.& Bennink, Maurice R.. Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats. Journal of Nutrition and Metabolism No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-465056
American Medical Association (AMA)
Rondini, Elizabeth A.& Bennink, Maurice R.. Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats. Journal of Nutrition and Metabolism. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-465056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465056