Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice
Joint Authors
Peralta, Humberto
Avila-Calderón, Eric Daniel
Sriranganathan, Nammalwar
Boyle, Stephen M.
Lopez-Merino, Ahidé
López-Villegas, Edgar Oliver
Contreras-Rodríguez, Araceli
Witonsky, Sharon
Jain, Neeta
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The outer membrane vesicles (OMVs) from smooth B.
melitensis 16 M and a derived rough mutant, VTRM1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice.
Proteomic analysis showed 29 proteins present in OMVs from B.
melitensis 16 M; some of them are well-known Brucella immunogens such as SOD, GroES, Omp31, Omp25, Omp19, bp26, and Omp16.
OMVs from a rough VTRM1 induced significantly higher expression of IL-12, TNFα, and IFNγ genes in bone marrow dendritic cells than OMVs from smooth strain 16 M.
Relative to saline control group, mice immunized intramuscularly with rough and smooth OMVs were protected from challenge with virulent strain B.
melitensis 16 M just as well as the group immunized with live strain B.
melitensis Rev1 (P<0.005).
Additionally, the levels of serum IgG2a increased in mice vaccinated with OMVs from rough strain VTRM1 consistent with the induction of cell-mediated immunity.
American Psychological Association (APA)
Avila-Calderón, Eric Daniel& Lopez-Merino, Ahidé& Jain, Neeta& Peralta, Humberto& López-Villegas, Edgar Oliver& Sriranganathan, Nammalwar…[et al.]. 2011. Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-465128
Modern Language Association (MLA)
Avila-Calderón, Eric Daniel…[et al.]. Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice. Clinical and Developmental Immunology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-465128
American Medical Association (AMA)
Avila-Calderón, Eric Daniel& Lopez-Merino, Ahidé& Jain, Neeta& Peralta, Humberto& López-Villegas, Edgar Oliver& Sriranganathan, Nammalwar…[et al.]. Characterization of Outer Membrane Vesicles from Brucella melitensis and Protection Induced in Mice. Clinical and Developmental Immunology. 2011. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-465128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465128