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Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia
Joint Authors
Bülte, M.
Abdulmawjood, A.
Fernández-Silva, J. A.
Source
Veterinary Medicine International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The objective of this study was the serological, bacteriological and molecular diagnosis, as well as the molecular characterization of Mycobacterium avium subsp.
paratuberculosis (Map) in adult cows of five Colombian dairy herds.
Serum samples were tested by an indirect absorbed enzyme–linked immunosorbent assay (ELISA-C).
All fecal samples were tested by pooled culture.
After that, fecal samples of Map positive pools were tested individually by culture and polymerase chain reaction (PCR).
In one herd, slurry and tissue samples from one animal were also taken and tested by PCR and culture.
Map isolates were analyzed by the Multilocus Short Sequence Repeat (MLSSR) and the Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) methods.
ELISA produced positive results in 1.8% (6/329) of the animals and 40% (2/5) of the herds.
Four fecal, two tissue, and two slurry samples from a herd were Map positive by culture and PCR.
MLSSR and MIRU-VNTR revealed two different strain profiles among eight Map isolates recovered.
This study reports the first molecular characterization of Map in one dairy herd in Colombia, the limitations for individual diagnosis of subclinical Map infections in cattle, and the usefulness of pooled fecal samples and environmental sampling for Map diagnosis.
American Psychological Association (APA)
Fernández-Silva, J. A.& Abdulmawjood, A.& Bülte, M.. 2011. Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia. Veterinary Medicine International،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-465135
Modern Language Association (MLA)
Fernández-Silva, J. A.…[et al.]. Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia. Veterinary Medicine International No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-465135
American Medical Association (AMA)
Fernández-Silva, J. A.& Abdulmawjood, A.& Bülte, M.. Diagnosis and Molecular Characterization of Mycobacterium avium subsp. paratuberculosis from Dairy Cows in Colombia. Veterinary Medicine International. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-465135
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465135