High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid : A Possible Extension toward Alzheimer's Disease Biomarker Development

Joint Authors

Nara, Kiyomitsu
Shirotani, Keiro
Arai, Hajime
Kitazume, Shinobu
Tohyama, Yuriko
Narimatsu, Hisashi
Futakawa, Satoshi
Kuno, Atsushi
Saito, Toshie
Hashimoto, Yasuhiro
Miyajima, Masakazu
Yuasa, Tatsuhiko
Hoshi, Kyoka

Source

International Journal of Alzheimer's Disease

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-08-17

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Diseases
Medicine

Abstract EN

We have established high-throughput lectin-antibody ELISAs to measure different glycans on transferrin (Tf) in cerebrospinal fluid (CSF) using lectins and an anti-transferrin antibody (TfAb).

Lectin blot and precipitation analysis of CSF revealed that PVL (Psathyrella velutina lectin) bound an unique N-acetylglucosamine-terminated N-glycans on “CSF-type” Tf whereas SSA (Sambucus sieboldiana agglutinin) bound α2,6-N-acetylneuraminic acid-terminated N-glycans on “serum-type” Tf.

PVL-TfAb ELISA of 0.5 μL CSF samples detected “CSF-type” Tf but not “serum-type” Tf whereas SSA-TfAb ELISA detected “serum-type” Tf but not “CSF-type” Tf, demonstrating the specificity of the lectin-TfAb ELISAs.

In idiopathic normal pressure hydrocephalus (iNPH), a senile dementia associated with ventriculomegaly, amounts of the SSA-reactive Tf were significantly higher than in non-iNPH patients, indicating that Tf glycan analysis by the high-throughput lectin-TfAb ELISAs could become practical diagnostic tools for iNPH.

The lectin-antibody ELISAs of CSF proteins might be useful for diagnosis of the other neurological diseases.

American Psychological Association (APA)

Shirotani, Keiro& Futakawa, Satoshi& Nara, Kiyomitsu& Hoshi, Kyoka& Saito, Toshie& Tohyama, Yuriko…[et al.]. 2011. High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid : A Possible Extension toward Alzheimer's Disease Biomarker Development. International Journal of Alzheimer's Disease،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-465165

Modern Language Association (MLA)

Shirotani, Keiro…[et al.]. High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid : A Possible Extension toward Alzheimer's Disease Biomarker Development. International Journal of Alzheimer's Disease No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-465165

American Medical Association (AMA)

Shirotani, Keiro& Futakawa, Satoshi& Nara, Kiyomitsu& Hoshi, Kyoka& Saito, Toshie& Tohyama, Yuriko…[et al.]. High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid : A Possible Extension toward Alzheimer's Disease Biomarker Development. International Journal of Alzheimer's Disease. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-465165

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-465165