Clonality and Resistome Analysis of KPC-Producing Klebsiella pneumoniae Strain Isolated in Korea Using Whole Genome Sequencing

Abstract EN

We analyzed the whole genome sequence and resistome of the outbreak Klebsiella pneumoniae strain MP14 and compared it with those of K.

pneumoniae carbapenemase- (KPC-) producing isolates that showed high similarity in the NCBI genome database.

A KPC-2-producing multidrug-resistant (MDR) K.

pneumoniae clinical isolate was obtained from a patient admitted to a Korean hospital in 2011.

The strain MP14 was resistant to all tested β-lactams including monobactam, amikacin, levofloxacin, and cotrimoxazole, but susceptible to tigecycline and colistin.

Resistome analysis showed the presence of β-lactamase genes including blaKPC-2, blaSHV-11, blaTEM-169, and blaOXA-9.

MP14 also possessed aac(6′-)Ib, aadA2, and aph(3′-)Ia as aminoglycoside resistance-encoding genes, mph(A) for macrolides, oqxA and oqxB for quinolone, catA1 for phenicol, sul1 for sulfonamide, and dfrA12 for trimethoprim.

Both SNP tree and cgMLST analysis showed the close relatedness with the KPC producers (KPNIH strains) isolated from an outbreak in the USA and colistin-resistant strains isolated in Italy.

The plasmid-scaffold genes in plasmids pKpQil, pKpQil-IT, pKPN3, or pKPN-IT were identified in MP14, KPNIH, and Italian strains.

The KPC-2-producing MDR K.

pneumoniae ST258 stain isolated in Korea was highly clonally related with MDR K.

pneumoniae strains from the USA and Italy.

Global spread of KPC-producing K.

pneumoniae is a worrying phenomenon.