The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver
Joint Authors
Latruffe, N.
Chamouton, J.
Martin, Pascal G.
Nicolas-Francès, V.
Bonzo, J. A.
Battle, M.
Mandard, Stephane
Clémencet, M. C.
Chevillard, G.
Duncan, S.
Pineau, T.
Hansmannel, F.
Gonzalez, Frank J.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-06
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
PPARα and HNF4α are nuclear receptors that control gene transcription by direct binding to specific nucleotide sequences.
Using transgenic mice deficient for either PPARα or HNF4α, we show that the expression of the peroxisomal 3-keto-acyl-CoA thiolase B (Thb) is under the dependence of these two transcription factors.
Transactivation and gel shift experiments identified a novel PPAR response element within intron 3 of the Thb gene, by which PPARα but not HNF4α transactivates.
Intriguingly, we found that HNF4α enhanced PPARα/RXRα transactivation from TB PPRE3 in a DNA-binding independent manner.
Coimmunoprecipitation assays supported the hypothesis that HNF4α was physically interacting with RXRα.
RT-PCR performed with RNA from liver-specific HNF4α-null mice confirmed the involvement of HNF4α in the PPARα-regulated induction of Thb by Wy14,643.
Overall, we conclude that HNF4α enhances the PPARα-mediated activation of Thb gene expression in part through interaction with the obligate PPARα partner, RXRα.
American Psychological Association (APA)
Chamouton, J.& Hansmannel, F.& Bonzo, J. A.& Clémencet, M. C.& Chevillard, G.& Battle, M.…[et al.]. 2011. The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver. PPAR Research،Vol. 2010, no. 2010, pp.1-17.
https://search.emarefa.net/detail/BIM-465187
Modern Language Association (MLA)
Chamouton, J.…[et al.]. The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver. PPAR Research No. 2010 (2010), pp.1-17.
https://search.emarefa.net/detail/BIM-465187
American Medical Association (AMA)
Chamouton, J.& Hansmannel, F.& Bonzo, J. A.& Clémencet, M. C.& Chevillard, G.& Battle, M.…[et al.]. The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver. PPAR Research. 2011. Vol. 2010, no. 2010, pp.1-17.
https://search.emarefa.net/detail/BIM-465187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465187