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Development of Stability Indicating LC Method for the Estimation of Tolperisone in Bulk and Pharmaceutical Dosage Form
Joint Authors
Patel, S. B.
Shah, Dimal
Baldania, Sunil L.
Chhalotiya, Usmangani K.
Bhatt, Kashyap K.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Science
Abstract EN
A rapid, specific, and sensitive reverse phase high performance liquid chromatographic method has been developed and validated for analysis of tolperisone in both bulk and pharmaceutical dosage form.
The HPLC method was performed with a reversed phase C18 SunFire column (250 mm × 4.6 mm i.d., 5 mm particle size), detection at 261 nm and a mixture of methanol, water and pH 7.5 adjusted by use of 1% solution of triethylamine (60 : 40) as mobile phase.
The flow rate was 1.0 mL min−1 and effluents were monitored at 261 nm.
The retention time of tolperisone was 4.8 min.
Tolperisone was subjected to acid and alkali hydrolysis, chemical oxidation, wet hydrolysis, dry heat degradation, and sunlight degradation.
The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values.
Stressed samples were assayed using developed LC method.
The proposed method was validated with respect to linearity, accuracy, precision, and robustness.
The method was successfully applied to the estimation of tolperisone in tablet dosage forms.
American Psychological Association (APA)
Chhalotiya, Usmangani K.& Bhatt, Kashyap K.& Shah, Dimal& Baldania, Sunil L.& Patel, S. B.. 2013. Development of Stability Indicating LC Method for the Estimation of Tolperisone in Bulk and Pharmaceutical Dosage Form. Journal of Applied Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-465189
Modern Language Association (MLA)
Chhalotiya, Usmangani K.…[et al.]. Development of Stability Indicating LC Method for the Estimation of Tolperisone in Bulk and Pharmaceutical Dosage Form. Journal of Applied Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-465189
American Medical Association (AMA)
Chhalotiya, Usmangani K.& Bhatt, Kashyap K.& Shah, Dimal& Baldania, Sunil L.& Patel, S. B.. Development of Stability Indicating LC Method for the Estimation of Tolperisone in Bulk and Pharmaceutical Dosage Form. Journal of Applied Chemistry. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-465189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465189