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Optimal Labeling Dose, Labeling Time, and Magnetic Resonance Imaging Detection Limits of Ultrasmall Superparamagnetic Iron-Oxide Nanoparticle Labeled Mesenchymal Stromal Cells
Joint Authors
Thomsen, Carsten
Hansen, Louise
Kastrup, Jens
Bhakoo, Kishore
Friis, Tina
Mathiasen, Anders Bruun
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Background.
Regenerative therapy is an emerging treatment modality.
To determine migration and retention of implanted cells, it is crucial to develop noninvasive tracking methods.
The aim was to determine ex vivo magnetic resonance imaging (MRI) detection limits of ultrasmall superparamagnetic iron-oxide (USPIO) labeled mesenchymal stromal cells (MSCs).
Materials and Methods.
248 gel-phantoms were constructed and scanned on a 1.5T MRI-scanner.
Phantoms contained human MSCs preincubated with USPIO nanoparticles for 2, 6, or 21 hours using 5 or 10 μg USPIO/105 MSCs.
In addition, porcine hearts were scanned after injection of USPIO labeled MSCs.
Results.
Using 21 h incubation time and 10 μg USPIO/105 MSCs, labeled cells were clearly separated from unlabeled cells on MRI using 250.000 (P<0.001), 500.000 (P=0.007), and 1.000.000 MSCs (P=0.008).
At lower incubation times and doses, neither labeled nor unlabeled cells could be separated.
In porcine hearts labeled, but not unlabeled, MSCs were identified on MRI.
Conclusions.
As few as 250.000 MSCs can be detected on MRI using 21 h incubation time and 10 μg USPIO/105 MSCs.
At lower incubation times and doses, several million cells are needed for MRI detection.
USPIO labeled cells can be visualized by MRI in porcine myocardial tissue.
American Psychological Association (APA)
Mathiasen, Anders Bruun& Hansen, Louise& Friis, Tina& Thomsen, Carsten& Bhakoo, Kishore& Kastrup, Jens. 2013. Optimal Labeling Dose, Labeling Time, and Magnetic Resonance Imaging Detection Limits of Ultrasmall Superparamagnetic Iron-Oxide Nanoparticle Labeled Mesenchymal Stromal Cells. Stem Cells International،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465200
Modern Language Association (MLA)
Mathiasen, Anders Bruun…[et al.]. Optimal Labeling Dose, Labeling Time, and Magnetic Resonance Imaging Detection Limits of Ultrasmall Superparamagnetic Iron-Oxide Nanoparticle Labeled Mesenchymal Stromal Cells. Stem Cells International No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-465200
American Medical Association (AMA)
Mathiasen, Anders Bruun& Hansen, Louise& Friis, Tina& Thomsen, Carsten& Bhakoo, Kishore& Kastrup, Jens. Optimal Labeling Dose, Labeling Time, and Magnetic Resonance Imaging Detection Limits of Ultrasmall Superparamagnetic Iron-Oxide Nanoparticle Labeled Mesenchymal Stromal Cells. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465200
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465200