Analysis of High-Throughput Flow Cytometry Data Using plateCore
Joint Authors
Hahne, Florian
Haaland, Perry
Brinkman, Ryan R.
Strain, Errol
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Flow cytometry (FCM) software packages from R/Bioconductor, such as flowCore and flowViz, serve as an open platform for development of new analysis tools and methods.
We created plateCore, a new package that extends the functionality in these core packages to enable automated negative control-based gating and make the processing and analysis of plate-based data sets from high-throughput FCM screening experiments easier.
plateCore was used to analyze data from a BD FACS CAP screening experiment where five Peripheral Blood Mononucleocyte Cell (PBMC) samples were assayed for 189 different human cell surface markers.
This same data set was also manually analyzed by a cytometry expert using the FlowJo data analysis software package (TreeStar, USA).
We show that the expression values for markers characterized using the automated approach in plateCore are in good agreement with those from FlowJo, and that using plateCore allows for more reproducible analyses of FCM screening data.
American Psychological Association (APA)
Strain, Errol& Hahne, Florian& Brinkman, Ryan R.& Haaland, Perry. 2009. Analysis of High-Throughput Flow Cytometry Data Using plateCore. Advances in Bioinformatics،Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-465385
Modern Language Association (MLA)
Strain, Errol…[et al.]. Analysis of High-Throughput Flow Cytometry Data Using plateCore. Advances in Bioinformatics No. 2009 (2009), pp.1-10.
https://search.emarefa.net/detail/BIM-465385
American Medical Association (AMA)
Strain, Errol& Hahne, Florian& Brinkman, Ryan R.& Haaland, Perry. Analysis of High-Throughput Flow Cytometry Data Using plateCore. Advances in Bioinformatics. 2009. Vol. 2009, no. 2009, pp.1-10.
https://search.emarefa.net/detail/BIM-465385
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465385