Spontaneous Periodontitis Development in Diabetic Rats Involves an Unrestricted Expression of Inflammatory Cytokines and Tissue Destructive Factors in the Absence of Major Changes in Commensal Oral Microbiota
Joint Authors
Spadella, César Tadeu
Gennaro, Gabriela
Assis, Gerson Francisco
Garlet, Gustavo Pompermaier
Claudino, Marcela
Cestari, Tania Mary
Source
Experimental Diabetes Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia is a main feature.
The objective was to evaluate the involvement of RAGE, inflammatory cytokines, and metalloproteinases in spontaneous periodontitis triggered by diabetes induction.
Immunohistochemical procedures for MMP-2, MMP-9, TNF-α, IL-1β, IL-6, RANKL, and RAGE were performed in rats after 1, 3, 6, 9, and 12 months of diabetes induction.
Total DNA was extracted from paraffin-embedded tissues and evaluated by Real-TimePCR for 16S total bacterial load and specific periodontopathogens.
Our data did not demonstrate differences in microbiological patterns between groups.
In diabetic groups, an increase in RAGE-positive cells was detected at 6, 9, and 12 months, while TNF-alpha-stained cells were more prevalent at 6 and 12 months.
In experimental groups, IL-β-positive cells were increased after 12 months, IL-6 stained cells were increased at 9 and 12 months, and RANKL-positive cells at 9 months.
Diabetes resulted in widespread expression of RAGE, followed by expression of proinflammatory mediators, without major alterations in oral microbial profile.
The pervasive expression of cytokines suggests that spontaneous periodontitis development may be independent of microbial stimulation and may be triggered by diabetes-driven imbalance of homeostasis.
American Psychological Association (APA)
Claudino, Marcela& Gennaro, Gabriela& Cestari, Tania Mary& Spadella, César Tadeu& Garlet, Gustavo Pompermaier& Assis, Gerson Francisco. 2012. Spontaneous Periodontitis Development in Diabetic Rats Involves an Unrestricted Expression of Inflammatory Cytokines and Tissue Destructive Factors in the Absence of Major Changes in Commensal Oral Microbiota. Experimental Diabetes Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-465455
Modern Language Association (MLA)
Claudino, Marcela…[et al.]. Spontaneous Periodontitis Development in Diabetic Rats Involves an Unrestricted Expression of Inflammatory Cytokines and Tissue Destructive Factors in the Absence of Major Changes in Commensal Oral Microbiota. Experimental Diabetes Research No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-465455
American Medical Association (AMA)
Claudino, Marcela& Gennaro, Gabriela& Cestari, Tania Mary& Spadella, César Tadeu& Garlet, Gustavo Pompermaier& Assis, Gerson Francisco. Spontaneous Periodontitis Development in Diabetic Rats Involves an Unrestricted Expression of Inflammatory Cytokines and Tissue Destructive Factors in the Absence of Major Changes in Commensal Oral Microbiota. Experimental Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-465455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465455