Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes

Joint Authors

Platas, Julia
Guillén, Maria Isabel
Alcaraz, Maria José
Mirabet, Vicente
del Caz, María Dolores Pérez
Gomar, Francisco

Source

Mediators of Inflammation

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-12-10

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Diseases

Abstract EN

Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability.

Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair.

We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration.

CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β.

In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase.

These effects may be dependent on the inhibition of nuclear factor-κB activation by CM.

Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease.

American Psychological Association (APA)

Platas, Julia& Guillén, Maria Isabel& del Caz, María Dolores Pérez& Gomar, Francisco& Mirabet, Vicente& Alcaraz, Maria José. 2013. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465475

Modern Language Association (MLA)

Platas, Julia…[et al.]. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-465475

American Medical Association (AMA)

Platas, Julia& Guillén, Maria Isabel& del Caz, María Dolores Pérez& Gomar, Francisco& Mirabet, Vicente& Alcaraz, Maria José. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465475

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-465475