Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes
Joint Authors
Platas, Julia
Guillén, Maria Isabel
Alcaraz, Maria José
Mirabet, Vicente
del Caz, María Dolores Pérez
Gomar, Francisco
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability.
Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair.
We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration.
CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β.
In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase.
These effects may be dependent on the inhibition of nuclear factor-κB activation by CM.
Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease.
American Psychological Association (APA)
Platas, Julia& Guillén, Maria Isabel& del Caz, María Dolores Pérez& Gomar, Francisco& Mirabet, Vicente& Alcaraz, Maria José. 2013. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465475
Modern Language Association (MLA)
Platas, Julia…[et al.]. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-465475
American Medical Association (AMA)
Platas, Julia& Guillén, Maria Isabel& del Caz, María Dolores Pérez& Gomar, Francisco& Mirabet, Vicente& Alcaraz, Maria José. Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-465475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465475