Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line
Joint Authors
Fang, Meixin
Jin, Changzhong
Wu, Nanping
Li, Jie
Wu, Lijuan
Cheng, Linfang
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is an important pattern recognition receptor on dendritic cells (DCs), and its expression shows significant cytological and histological specificity, being interleukine-4 (IL-4) dependent.
The signaling pathways through which IL-4 regulates expression of DC-SIGN are still unclear.
We used phorbol 12-myristate 13-acetate- (PMA-) differentiated THP-1 cells as the in vitro model of monocyte/macrophage cells to study the signaling pathways involved in IL-4-regulated expression of DC-SIGN.
We found that a high expression of DC-SIGN could be induced by IL-4 at the levels of mRNA and cell surface protein.
Upregulated expression of DC-SIGN was almost completely blocked by the specific inhibitor of ERK pathway, and partly reduced by the specific inhibitors of JAK-STAT and NF-κB pathways.
The activation of the three signaling pathways was directly confirmed by testing the phosphorylation of protein kinase within the cytoplasm and nucleus over time.
The analysis of cis-acting elements of DC-SIGN promoter showed that the activity of DC-SIGN promoter without Ets-1 transcription factors binding site almost completely disappeared.
Our results demonstrated that multiple signaling pathways are involved in IL-4 induced high expression of DC-SIGN on THP-1 cells, in which ERK pathway is the main signaling pathway and mediated by the Ets-1 transcription factors binding site.
American Psychological Association (APA)
Jin, Changzhong& Wu, Lijuan& Li, Jie& Fang, Meixin& Cheng, Linfang& Wu, Nanping. 2012. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-465485
Modern Language Association (MLA)
Jin, Changzhong…[et al.]. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-465485
American Medical Association (AMA)
Jin, Changzhong& Wu, Lijuan& Li, Jie& Fang, Meixin& Cheng, Linfang& Wu, Nanping. Multiple Signaling Pathways Are Involved in the Interleukine-4 Regulated Expression of DC-SIGN in THP-1 Cell Line. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-465485
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465485