Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain : A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors
Joint Authors
Mothe, Beatriz
Fernández-Lloris, Raquel
Gatell, Josep Maria
Cardona, Pere-Joan
Joseph, Joan
Pezzat, Elías
Saubi, Narcís
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Mycobacterium bovis Bacillus Calmette-Guérin (BCG) as a live vector of recombinant bacterial vaccine is a promising system to be used.
In this study, we evaluate the disrupted expression of heterologous HIV-1gp120 gene in BCG Pasteur host strain using replicative vectors pMV261 and pJH222.
pJH222 carries a lysine complementing gene in BCG lysine auxotrophs.
The HIV-1 gp120 gene expression was regulated by BCG hsp60 promoter (in plasmid pMV261) and Mycobacteria spp.
α-antigen promoter (in plasmid pJH222).
Among 14 rBCG:HIV-1gp120 (pMV261) colonies screened, 12 showed a partial deletion and two showed a complete deletion.
However, deletion was not observed in all 10 rBCG:HIV-1gp120 (pJH222) colonies screened.
In this study, we demonstrated that E.
coli/Mycobacterial expression vectors bearing a weak promoter and lysine complementing gene in a recombinant lysine auxotroph of BCG could prevent genetic rearrangements and disruption of HIV 1gp120 gene expression, a key issue for engineering Mycobacterial based vaccine vectors.
American Psychological Association (APA)
Joseph, Joan& Fernández-Lloris, Raquel& Pezzat, Elías& Saubi, Narcís& Cardona, Pere-Joan& Mothe, Beatriz…[et al.]. 2010. Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain : A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-465522
Modern Language Association (MLA)
Joseph, Joan…[et al.]. Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain : A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-465522
American Medical Association (AMA)
Joseph, Joan& Fernández-Lloris, Raquel& Pezzat, Elías& Saubi, Narcís& Cardona, Pere-Joan& Mothe, Beatriz…[et al.]. Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain : A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-465522
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465522