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Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population
Joint Authors
Sheng, Quanhu
Jia, Weiping
Wang, Congrong
Zeng, Rong
Fang, Qichen
Jiang, Feng
Li, Rongxia
Hu, Cheng
Bao, Yu-Qian
Xiang, Kunsan
Zhang, Rong
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Type 2 diabetes and its chronic complications have become a worldwide epidemic nowadays.
However, its molecular mechanism is still unknown.
We have previously identified a novel variant rs12742393 of NOS1AP for type 2 diabetes susceptibility in the Chinese population.
In this study, we analyzed the total serum profiling among three genotypes of rs12742393 to discover potential crosstalk under the variant and the disease through proteomic analyses for the first time.
We used OFFGEL peptide fractionation, LC-MS/MS analysis, and label-free quantification to profile the fasting human serum samples of the genotypes in rs12742393 (n=4, for CC, AC, and AA, resp.).
Four proteins were identified, including apoA4, alpha1-ACT, HABP2, and keratin 10, with blood levels changed significantly between CC and AA homozygotes of rs12742393.
Compared with AA group, the levels of apoA4 increased (P=0.000265), whereas the concentration of alpha1-ACT, HABP2, and keratin 10 decreased in CC group (P=0.011116, 0.021175, and 0.015661, resp.).
Then we selected additional fasting serum samples for ELISA and western blot validation.
However, no significant differences were identified by neither ELISA nor western blot (P>0.05).
The protein profiling changes between the genotypes of rs12742393 indicated that this SNP might play a role in the development of type 2 diabetes.
American Psychological Association (APA)
Jiang, Feng& Wang, Congrong& Li, Rongxia& Sheng, Quanhu& Hu, Cheng& Zhang, Rong…[et al.]. 2013. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-465540
Modern Language Association (MLA)
Jiang, Feng…[et al.]. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. Journal of Diabetes Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-465540
American Medical Association (AMA)
Jiang, Feng& Wang, Congrong& Li, Rongxia& Sheng, Quanhu& Hu, Cheng& Zhang, Rong…[et al.]. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-465540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465540