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Experimentally Approaching the ICU : Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis
Joint Authors
Drechsler, Susanne
van Griensven, Martijn
Osuchowski, Marcin F.
Redl, Heinz
Bahrami, Soheyl
Weixelbaumer, Katrin M.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-01-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
To simulate and monitor the evolution of posttraumatic sepsis in mice, we combined a two-hit model of trauma/hemorrhage (TH) followed by polymicrobial sepsis with repetitive blood sampling.
Anesthetized mice underwent femur fracture/sublethal hemorrhage and cecal ligation and puncture (CLP) 48 h later.
To monitor outcome-dependent changes in circulating cells/biomarkers, mice were sampled daily (facial vein) for 7 days and retrospectively divided into either dead (DIE) or surviving (SUR) by post-CLP day 7.
Prior to CLP, AST was 3-fold higher in DIE, while all other post-TH changes were similar between groups.
There was a significant post-CLP intergroup separation.
In SUR, RBC and Hb were lower, platelets and neutrophils higher, and lymphocytes mixed compared to DIE.
In DIE, all organ function markers except glucose (decrease) were few folds higher compared to SUR.
In summary, the combination of daily monitoring with an adequate two-hit model simulates the ICU setting, allows insight into outcome-based responses, and can identify biomarkers indicative of death in the acute posttraumatic sepsis in mice.
American Psychological Association (APA)
Drechsler, Susanne& Weixelbaumer, Katrin M.& Redl, Heinz& van Griensven, Martijn& Bahrami, Soheyl& Osuchowski, Marcin F.. 2011. Experimentally Approaching the ICU : Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-465560
Modern Language Association (MLA)
Drechsler, Susanne…[et al.]. Experimentally Approaching the ICU : Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-465560
American Medical Association (AMA)
Drechsler, Susanne& Weixelbaumer, Katrin M.& Redl, Heinz& van Griensven, Martijn& Bahrami, Soheyl& Osuchowski, Marcin F.. Experimentally Approaching the ICU : Monitoring Outcome-Based Responses in the Two-Hit Mouse Model of Posttraumatic Sepsis. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-465560
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465560