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Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis
Joint Authors
Cheng, Chih-Yu
Huang, Chen-Ji
Peng, Hwei-Ling
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-29
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In order to enhance the sensitivity of diagnosis, a recombinant clone containing domain I of HCV core (amino acid residues 1 to 123) was subjected to random mutagenesis.
Five mutants with higher sensitivity were obtained by colony screening of 616 mutants using reverse ELISA.
Sequence analysis of these mutants revealed alterations focusing on W84, P95, P110, or V129.
The inclusion bodies of these recombinant proteins overexpressed in E.
coli BL21(DE3) were subsequently dissolved using 6 M urea and then refolded by stepwise dialysis.
Compared to the unfolded wild-type antigen, the refolded M3b antigen (W84S, P110S and V129L) exhibited an increase of 66% antigenicity with binding capacity of 0.96 and affinity of 113 μM−1.
Moreover, the 33% decrease of the production demand suggests that M3b is a potential substitute for anti-HCV antibody detection.
American Psychological Association (APA)
Huang, Chen-Ji& Peng, Hwei-Ling& Cheng, Chih-Yu. 2011. Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-465668
Modern Language Association (MLA)
Huang, Chen-Ji…[et al.]. Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-465668
American Medical Association (AMA)
Huang, Chen-Ji& Peng, Hwei-Ling& Cheng, Chih-Yu. Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-465668
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465668