Plant Regeneration and Cellular Behaviour Studies in Celosia cristata Grown In Vivo and In Vitro
Joint Authors
Taha, Rosna Mat
Wafa, Sharifah Nurashikin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined.
It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L BAP, with the best response showed by shoot explants.
In vitro flowering was observed on MS basal medium after six weeks.
The occurrence of somaclonal variation and changes in cellular behavior from in vivo and in vitro grown plants were investigated through cytological studies and image analysis.
It was observed that Mitotic Index (MI), mean chromosome numbers, and mean nuclear to cell area ratio of in vitro root meristem cells were slightly higher compared to in vivo values.
However, in vitro plants produced lower mean cell areas but higher nuclear areas when compared to in vivo plants.
Thus, no occurrence of somaclonal variation was detected, and this was supported by morphological features of the in vitro plants.
American Psychological Association (APA)
Taha, Rosna Mat& Wafa, Sharifah Nurashikin. 2012. Plant Regeneration and Cellular Behaviour Studies in Celosia cristata Grown In Vivo and In Vitro. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465715
Modern Language Association (MLA)
Taha, Rosna Mat& Wafa, Sharifah Nurashikin. Plant Regeneration and Cellular Behaviour Studies in Celosia cristata Grown In Vivo and In Vitro. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-465715
American Medical Association (AMA)
Taha, Rosna Mat& Wafa, Sharifah Nurashikin. Plant Regeneration and Cellular Behaviour Studies in Celosia cristata Grown In Vivo and In Vitro. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465715