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A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine) : A Validation Study and Application
Joint Authors
Grotzkyj Giorgi, Margherita
Bonner, Adrian B.
Martin, Colin
Howland, Kevin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine).
Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol.
Total run time was 35 minutes.
Detection was performed with diode array set at 280 nm.
Each vitamin was quantitatively determined at its maximum wavelength.
Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males).
Interday and intraday precision were <4% and <7%, respectively, for all vitamins.
Recovery percentages ranged from 93% to 100%.
American Psychological Association (APA)
Grotzkyj Giorgi, Margherita& Howland, Kevin& Martin, Colin& Bonner, Adrian B.. 2012. A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine) : A Validation Study and Application. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465748
Modern Language Association (MLA)
Grotzkyj Giorgi, Margherita…[et al.]. A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine) : A Validation Study and Application. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-465748
American Medical Association (AMA)
Grotzkyj Giorgi, Margherita& Howland, Kevin& Martin, Colin& Bonner, Adrian B.. A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine) : A Validation Study and Application. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465748