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Monoclonal Antibody Targeting Neutralizing Epitope on H5N1 Influenza Virus of Clade 1 and 0 for Specific H5 Quantification
Joint Authors
Source
Influenza Research and Treatment
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
H5N1 influenza viruses cause high mortality in avian and mammalian species, including humans.
Antigenic drift in H5 sequence poses challenges in the development of vaccine and therapeutic antibody.
In this study, a monoclonal antibody 11G12 was produced from inactivated H5N1 immunized mice.
Results from IFA, ELISA, HI, and virus neutralization indicated that Mab 11G12 can specifically recognize and neutralize H5 type hemagglutinin from clade 1 and 0 without any cross-reaction to any other clades of H5N1 viruses.
Mab 11G12 was used to differentiate and quantify the expression of H5N1 strain A/VietNam/1203/04 from a trivalent vaccine mix in ELISA.
Sequencing of escape mutants identified that Mab 11G12 targets a major neutralizing epitope of influenza H5 hemagglutinin.
The study indicated that some major neutralizing epitopes in H5s of early strains were mutated due to antigenic drift.
American Psychological Association (APA)
Fang, H. E.& Kwang, Jimmy. 2013. Monoclonal Antibody Targeting Neutralizing Epitope on H5N1 Influenza Virus of Clade 1 and 0 for Specific H5 Quantification. Influenza Research and Treatment،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-465825
Modern Language Association (MLA)
Fang, H. E.& Kwang, Jimmy. Monoclonal Antibody Targeting Neutralizing Epitope on H5N1 Influenza Virus of Clade 1 and 0 for Specific H5 Quantification. Influenza Research and Treatment No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-465825
American Medical Association (AMA)
Fang, H. E.& Kwang, Jimmy. Monoclonal Antibody Targeting Neutralizing Epitope on H5N1 Influenza Virus of Clade 1 and 0 for Specific H5 Quantification. Influenza Research and Treatment. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-465825
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465825