The Study of Fetal Rat Model of Intra-Amniotic Isoproterenol Injection Induced Heart Dysfunction and Phenotypic Switch of Contractile Proteins
Joint Authors
Mu, Dezhi
Zhou, Kaiyu
Wang, Chuan
Li, Yifei
Fang, Jie
Hua, Yimin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
To establish a reliable isoproterenol induced heart dysfunction fetal rat model and understand the switches of contractile proteins, 45 pregnant rats were divided into 15 mg/kg-once, 15 mg/kg-twice, sham-operated once, sham-operated twice, and control groups.
And 18 adult rats were divided into isoproterenol-treated and control groups.
H&E staining, Masson staining, and transmission electron microscope were performed.
Apoptotic rate assessed by TUNEL analysis and expressions of ANP, BNP, MMP-2, and CTGF of hearts were measured.
Intra-amniotic injections of isoproterenol were supplied on E14.5 and E15.5 for fetuses and 7-day continuous intraperitoneal injections were performed for adults.
Then echocardiography was performed with M-mode view assessment on E18.5 and 6 weeks later, respectively.
Isoproterenol twice treated fetuses exhibited significant changes in histological evaluation, and mitochondrial damages were significantly severe with increased apoptotic rate.
ANP and BNP increased and that of MMP-2 increased in isoproterenol twice treated group compared to control group, without CTGF.
The isoforms transition of troponin I and myosin heavy chain of fetal heart dysfunction were opposite to adult procedure.
The administration of intra-amniotic isoproterenol to fetal rats could induce heart dysfunction and the regulation of contractile proteins of fetuses was different from adult procedure.
American Psychological Association (APA)
Li, Yifei& Fang, Jie& Hua, Yimin& Wang, Chuan& Mu, Dezhi& Zhou, Kaiyu. 2014. The Study of Fetal Rat Model of Intra-Amniotic Isoproterenol Injection Induced Heart Dysfunction and Phenotypic Switch of Contractile Proteins. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-465828
Modern Language Association (MLA)
Li, Yifei…[et al.]. The Study of Fetal Rat Model of Intra-Amniotic Isoproterenol Injection Induced Heart Dysfunction and Phenotypic Switch of Contractile Proteins. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-465828
American Medical Association (AMA)
Li, Yifei& Fang, Jie& Hua, Yimin& Wang, Chuan& Mu, Dezhi& Zhou, Kaiyu. The Study of Fetal Rat Model of Intra-Amniotic Isoproterenol Injection Induced Heart Dysfunction and Phenotypic Switch of Contractile Proteins. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-465828
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465828