![](/images/graphics-bg.png)
Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia
Joint Authors
Lee, Kang-Moon
Paik, Chang Nyol
Noh, Seung June
Kim, Young Wook
Lee, Yun Kyung
Jung, Ji Han
Kim, Jin Su
Chung, Woo Chul
Lee, Kyeong Soo
Kim, Hye Ji
Source
Gastroenterology Research and Practice
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation.
In this study, we measured LINE-1 methylation level according to genetic instability and evaluated the effect of Helicobacter pylori infection on genetic instability in gastric epithelial dysplasia.
Methods.
Total 100 tissue samples of gastric epithelial dysplasia were analyzed.
Seven loci that linked to tumor suppressor genes were used to identify significant structural chromosomal aberrations.
Microsatellite status was investigated for two different microsatellite marker loci (BAT25 and BAT26).
Also, we measured LINE-1 methylation level by combined bisulfite restriction analysis (COBRA-LINE-1) method.
Results.
There were no significant differences of LINE-1 methylation level according to chromosomal/microsatellite instability and H.
pylori state.
In the dysplastic lesions with H.
pylori infection, LINE-1 methylation level of MSI lesion was significantly lower than that of microsatellite stable (MSS) lesion (40.23±4.47 versus 43.90±4.81%, P<0.01).
Conclusions.
In gastric epithelial dysplasia with H.
pylori infection, MSI is correlated with reduced LINE-1 methylation level.
Coexistence of H.
pylori infection and MSI might be a driving force of gastric carcinogenesis.
American Psychological Association (APA)
Kim, Jin Su& Chung, Woo Chul& Lee, Kang-Moon& Paik, Chang Nyol& Lee, Kyeong Soo& Kim, Hye Ji…[et al.]. 2012. Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia. Gastroenterology Research and Practice،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-465845
Modern Language Association (MLA)
Kim, Jin Su…[et al.]. Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia. Gastroenterology Research and Practice No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-465845
American Medical Association (AMA)
Kim, Jin Su& Chung, Woo Chul& Lee, Kang-Moon& Paik, Chang Nyol& Lee, Kyeong Soo& Kim, Hye Ji…[et al.]. Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia. Gastroenterology Research and Practice. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-465845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465845