The Design of a Quantitative Western Blot Experiment
Joint Authors
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample.
Although there have been significant advances in both the imaging and reagent technologies to improve sensitivity, dynamic range of detection, and the applicability of multiplexed target detection, the basic technique has remained essentially unchanged.
In the past, western blotting was used simply to detect a specific target protein in a complex mixture, but now journal editors and reviewers are requesting the quantitative interpretation of western blot data in terms of fold changes in protein expression between samples.
The calculations are based on the differential densitometry of the associated chemiluminescent and/or fluorescent signals from the blots and this now requires a fundamental shift in the experimental methodology, acquisition, and interpretation of the data.
We have recently published an updated approach to produce quantitative densitometric data from western blots (Taylor et al., 2013) and here we summarize the complete western blot workflow with a focus on sample preparation and data analysis for quantitative western blotting.
American Psychological Association (APA)
Taylor, Sean C.& Posch, Anton. 2014. The Design of a Quantitative Western Blot Experiment. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-465910
Modern Language Association (MLA)
Taylor, Sean C.& Posch, Anton. The Design of a Quantitative Western Blot Experiment. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-465910
American Medical Association (AMA)
Taylor, Sean C.& Posch, Anton. The Design of a Quantitative Western Blot Experiment. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-465910
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465910