In Situ Expression of Regulatory Cytokines by Heart Inflammatory Cells in Chagas' Disease Patients with Heart Failure
Joint Authors
Teixeira, Vicente de Paula Antunes
Pereira, Sanívia Aparecida de Lima
Romano, Audrey
Reis, Marlene Antônia dos
Rodrigues, Denise Bertulucci Rocha
Rodrigues, Virmondes
Tostes Junior, Sebastião
Source
Clinical and Developmental Immunology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chagas' disease is caused by the protozoan parasite Trypanosoma cruzi.
The immune system plays an important role in the reduction of parasite load, but may also contribute to the development of lesions observed during the chronic phase of the disease.
We analyzed cytokines produced by inflammatory heart cells in 21 autopsy samples obtained from patients with Chagas' disease divided according to the presence or absence of heart failure (HF).
Left ventricular sections were analyzed by immunohistochemistry using antibodies against human IL-4, IFN-γ, TGF-β, TNF-α, and NOS2.
In situ mRNA expression was quantified by a Low Density Array.
The number of IFN-γ-positive cells was significantly higher than IL-4 positive cells.
TNF-α, TGF-β and NOS2 were detected in 65%, 62% and 94% of samples respectively.
There was an association between TNF-α-producing cells and the presence of HF.
Subjects with HF presented higher levels of STAT4 mRNA, whereas FoxP3 and STAT6 levels were similar in the two groups.
A Th1 cytokine pattern predominated in the cardiac inflammatory cell infiltrate of Chagas’ disease patients associated with HF.
High degree of fibrosis was associated with low NOS2 expression.
These results support the idea that Th1 immune responses are involved in heart lesions of Chagas' disease patients.
American Psychological Association (APA)
Rodrigues, Denise Bertulucci Rocha& Reis, Marlene Antônia dos& Romano, Audrey& Pereira, Sanívia Aparecida de Lima& Teixeira, Vicente de Paula Antunes& Tostes Junior, Sebastião…[et al.]. 2012. In Situ Expression of Regulatory Cytokines by Heart Inflammatory Cells in Chagas' Disease Patients with Heart Failure. Clinical and Developmental Immunology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-465922
Modern Language Association (MLA)
Rodrigues, Denise Bertulucci Rocha…[et al.]. In Situ Expression of Regulatory Cytokines by Heart Inflammatory Cells in Chagas' Disease Patients with Heart Failure. Clinical and Developmental Immunology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-465922
American Medical Association (AMA)
Rodrigues, Denise Bertulucci Rocha& Reis, Marlene Antônia dos& Romano, Audrey& Pereira, Sanívia Aparecida de Lima& Teixeira, Vicente de Paula Antunes& Tostes Junior, Sebastião…[et al.]. In Situ Expression of Regulatory Cytokines by Heart Inflammatory Cells in Chagas' Disease Patients with Heart Failure. Clinical and Developmental Immunology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-465922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465922