A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera
Joint Authors
Singh, Dushyant
Khan, Mohd Y.
Jamal, Farrukh
Pandey, Prabhash K.
Singh, Sangram
Source
Biochemistry Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Helicoverpa armigera is one of the major devastating pests of crop plants.
In this context a serine peptidase inhibitor purified from the seeds of Butea monosperma was evaluated for its effect on developmental physiology of H.
armigera larvae.
B.
monosperma peptidase inhibitor on 12% denaturing polyacrylamide gel electrophoresis exhibited a single protein band of ~14 kDa with or without reduction.
In vitro studies towards total gut proteolytic enzymes of H.
armigera and bovine trypsin indicated measurable inhibitory activity.
B.
monosperma peptidase inhibitor dose for 50% mortality and weight reduction by 50% were 0.5% w/w and 0.10% w/w, respectively.
The IC50 of B.
monosperma peptidase inhibitor against total H.
armigera gut proteinases activity was 2.0 µg/mL.
The larval feeding assays suggested B.
monosperma peptidase inhibitor to be toxic as reflected by its retarded growth and development, consequently affecting fertility and fecundity of pest and prolonging the larval-pupal duration of the insect life cycle of H.
armigera.
Supplementing B.
monosperma peptidase inhibitor in artificial diet at 0.1% w/w, both the efficiencies of conversion of ingested as well as digested food were downregulated, whereas approximate digestibility and metabolic cost were enhanced.
The efficacy of Butea monosperma peptidase inhibitor against progressive growth and development of H.
armigera suggest its usefulness in insect pest management of food crops.
American Psychological Association (APA)
Pandey, Prabhash K.& Singh, Dushyant& Singh, Sangram& Khan, Mohd Y.& Jamal, Farrukh. 2014. A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera. Biochemistry Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-465934
Modern Language Association (MLA)
Pandey, Prabhash K.…[et al.]. A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera. Biochemistry Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-465934
American Medical Association (AMA)
Pandey, Prabhash K.& Singh, Dushyant& Singh, Sangram& Khan, Mohd Y.& Jamal, Farrukh. A Nonhost Peptidase Inhibitor of ~14 kDa from Butea monosperma (Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology of Helicoverpa armigera. Biochemistry Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-465934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-465934